A new actinomycete strain SK4-6 producing secondary metabolite effective against methicillin-resistant Staphylococcus aureus |
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| Authors: | Rabah Forar Laidi Mohammed Sifour Mahmoud Sakr Hocine Hacene |
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| Affiliation: | (1) Microbiology Department, Faculty of Sciences, Houari Boumediene University of Science and Technology, Bab-Azzouar, Algiers, Algeria;(2) Microbiology Department, Faculty of Sciences, University of Jijel, Jijel, Algeria;(3) Plant Molecular Genetics Group, Nobel Project, National Research Centre (NRC), Dokki, Cairo, Egypt |
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| Abstract: | A new actinomycete strain designated SK4-6, was isolated. This organism exhibited strong activity against bacteria including methicillin-resistant Staphylococcus aureus and Micrococcus luteus, in addition to the causative agents of Candidiasis and Aspergillosis diseases, Candida albicans and Aspergillus species respectively. Morphological and chemical studies indicated that this organism belongs to the genus Streptomyces. Analysis of the 16S rRNA sequence of strain SK4-6 showed a high similarity, 99%, with S. qinlingensis. Optimization of cultural conditions was carried out using Plackett–Burman statistical design where seven variables were examined. Starch, pH and inoculum size showed a positive effect on the production of the active substances, however, sucrose, (NH4)2SO4 and yeast extract repress the production. |
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| Keywords: | Actinomycete Medium optimization Plackett– Burman design Strain SK4-6 Streptomyces |
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