Initial steps in pyrimidine synthesis in Ehrlich ascites carcinoma |
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| Authors: | W T Shoaf M E Jones |
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| Affiliation: | 1. Department of Biochemistry, School of Medicine, University of North Carolina, Chapel Hill, N. C. 27514 USA;2. Department of Biochemistry, School of Medicine, University of Southern California, Los Angeles, California 90033 USA;1. Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, USA;2. Program in Chemical Engineering, University of California, Santa Barbara, CA, USA;3. Program in Biomolecular Sciences and Engineering, University of California, Santa Barbara, CA, USA;1. BioTechnology Institute, University of Minnesota, St. Paul, MN, United States;2. School of Science, University of Waikato, Hamilton, New Zealand;3. Department of Biochemistry, University of Otago, Dunedin, New Zealand;1. Huck Institutes of Life Sciences, Pennsylvania State University, University Park, PA 16802, USA;2. Department of Chemistry, Pennsylvania State University, University Park, PA 16802, USA;3. Department of Molecular Biology and Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA;1. Aurigene Discovery Technologies (M) Sdn. Bhd., Level 2, Research Management and Innovation Complex, University of Malaya, 50603 Kuala Lumpur, Malaysia;2. Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia;3. Institute of Transdisciplinary Health Sciences and Technology (TDU) #74/2, Jarakabande Kaval, Post Attur via Yelahanka, Bangalore, 560 064 Karnataka, India;4. Aurigene Discovery Technologies Limited, 39-40, KIADB Industrial Area, Electronic City Phase II, Hosur Road, Bangalore, 560100 Karnataka, India |
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| Abstract: | The pyrimidine specific carbamyl phosphate synthetase (CPSase) and aspartate transcarbamylase (ATCase) of the Ehrlich ascites carcinoma have been found to exist as a complex with a molecular weight of 750,000–850,000 on sucrose gradients in the presence of 30% dimethyl sulfoxide (DMSO). In the presence of 10% DMSO, the activities separate and the remaining CPSase activity has a molecular weight of 150,000–200,000 while the ATCase now exists as two peaks of activity, with molecular weights of 300,00–400,000 and 525,000–700,000, depending on the exact conditions of the gradient. Dihydroorotase activity was found to co-sediment also with the two ATCase peaks under these conditions. It appears then that ATCase can be associated with CPSase and DHOase and that these first three enzymes of the pyrimidine biosynthetic pathway may exist as a complex. |
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