Regulation of phosphoglycerate mutase in developing forespores and dormant spores ofBacillus megaterium by thein vivo levels of phosphoglycerate mutase inhibitor |
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Authors: | Ravendra Pal Singh |
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Affiliation: | (1) Department of Biochemistry, University of Connecticut, Health Center, 06032 Farmington, Conn., USA;(2) Present address: Biochemistry Division, Regional Research Laboratory, 785 006 Jorhat |
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Abstract: | Bacillus megaterium accumulated 3-phosphoglycerate during sporulation which was utilized during spore germination. During sporulation a protein was synthesized before or at the start of 3-phosphoglycerate accumulation inside the developing spores about 1.5 h before dipicolinic acid accumulation. This protein has an affinity for Mn2+ and other divalent metal ions and inhibits phosphoglycerate mutase activity which has been shown to require Mn2+ However, the levels of the inhibitor decreased considerably (75–85%) during spore germination. No appreciable amount of the inhibitor was detected in the vegetable cell and mother cell compartment; however, the forespore compartment possesses an activity comparable to that of dormant spores. The partially purified inhibitor has a molecular weight of 11,000 and possesses both high and low affinity binding sites for Mn2+ and Ca2+ as determined by Scatchard plot analysis. |
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Keywords: | Bacillus megaterium 3-phosphoglyceric acid phosphoglycerate mutase mutase inhibitor dipicolinic acid |
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