| 一株将甜菊苷转化为甜茶甙的细菌鉴定及转化特性 |
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| 引用本文： | 姜中玉,陈育如,刘虎. 一株将甜菊苷转化为甜茶甙的细菌鉴定及转化特性[J]. 微生物学报, 2011, 51(1): 43-49 |
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| 作者姓名： | 姜中玉 陈育如 刘虎 |
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| 作者单位： | 1. 南京师范大学生命科学学院,江苏省生物多样性与生物技术重点实验室,江苏省微生物资源工程技术研究中心,江苏省功能微生物与功能基因组学重点实验室,南京,210046
2. 南京师范大学生命科学学院,江苏省生物多样性与生物技术重点实验室,江苏省微生物资源工程技术研究中心,江苏省功能微生物与功能基因组学重点实验室,南京,210046;南京师范大学泰州学院生物技术系,制药工程系,泰州,225300 |
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| 基金项目： | 林业公益性行业科研专项基金（200904055） |
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| 摘 要： | 摘要：【目的】筛选一株对甜菊苷具有特异转化性能的细菌,并对该菌及转化产物进行鉴定,探讨转化酶及酶对甜菊苷的转化特性。【方法】通过16S rDNA序列分析,构建该菌系统进化树,结合菌体形态及菌落特征,确立该菌系统发育学地位。通过高效液相色谱（HPLC）及液质联用（LC－MS）法检测并鉴定转化产物。用菌液直接对甜菊苷进行转化以研究菌的转化能力。用静息细胞、胞外液和胞内液对甜菊糖分别转化法,确定转化酶与菌体的关系,并用该酶液进行转化特性研究。【结果】该菌株与黄杆菌属的16S rDNA序列相似性为99%,结合菌体
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| 关 键 词： | 关键词：黄杆菌 甜菊苷 甜茶甙 胞内酶 生物转化 |
| 收稿时间： | 2010-05-01 |
| 修稿时间： | 2010-06-03 |
Identification and biotransformation properties of a bacterium that converts stevioside into rubusoside |
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| Zhongyu Jiang,Yuru Chen and Hu Liu. Identification and biotransformation properties of a bacterium that converts stevioside into rubusoside[J]. Acta microbiologica Sinica, 2011, 51(1): 43-49 |
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| Authors: | Zhongyu Jiang Yuru Chen Hu Liu |
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| Affiliation: | Jiangshu Engineering and Technology Research Center for Microbiology Resources, Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, China;Jiangshu Engineering and Technology Research Center for Microbiology Resources, Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, China;Jiangshu Engineering and Technology Research Center for Microbiology Resources, Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, China |
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| Abstract: | [Objective]The purpose of our study was to separate and identify a bacillus that could convert stevioside specifically.Then we identified the conversion product and studied the conversion capability of the bacillus.We also studied the enzyme with conversion capability and the conversion characteristic of the enzyme.[Methods]The bacillus was identified on the basis of morphology features and 16S rDNA sequence analysis.Phylogenetic tree was constructed to determine its taxonomic status.The product was detecte... |
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| Keywords: | Keywords: Chryseobacterium sp. stevioside rubusoside intracellular enzymes biotransformation |
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