An increase in intracellular pH is a general response of promonocytic cells to differentiating agents |
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Authors: | A Ladoux C Damais I Krawice J P Abita C Frelin |
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Institution: | INSERM U 204, Hopital Saint Louis, Paris, France. |
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Abstract: | Intracellular pH (pHi) was measured in HL60 and U937 cells before and after differentiation into monocyte-macrophage like cells. 12-O-Tetradecanoyl phorbol-13-acetate (PMA), butyrate, interferon, retinoic acid and 1,25-dihydroxyvitamin D3 all increased pHi. The increases elicited were rapid with PMA, much slower with retinoic acid and interferon and still slower with 1,25-dihydroxyvitamin D3. Increases in pHi are due to an activation of the Na+/H+ exchange system. High pHi values are unlikely to serve as an early intracellular signal for initiating monocytic differentiation. |
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