A Study of the Aminoacyl-sRNA Synthetases of Phaseolus vulgaris in Relation to Germination |
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| Authors: | Anderson J W Fowden L |
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| Affiliation: | Botany Department, University College London, Gower St., London WC1, England. |
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| Abstract: | Optimum conditions for the extraction and assay by ATP-pyrophosphate exchange of the aminoacyl-sRNA synthetases of the various tissues of french bean (Phaseolus vulgaris) seeds and seedlings are described. Extracts of plumules, after passage through Sephadex G-25, were assayed for synthetase activity using an amino acid mixture as substrate, when a 30 to 100-fold stimulation of exchange above the endogenous level was obtained. This marked enhancement of exchange by added amino acids is largely attributed to the use of dilute extracts. |
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