人工诱导泥鳅与大鳞副泥鳅雄核发育单倍体子代的RAPD分析

用３０个经过筛选的随机引物对３组泥鳅雄核发育单倍体、２组大鳞副尼鳅雄核发育单倍体及其相应亲本进行了ＲＡＰＤ分析。结果表明，雄核发育倍体子代与其父本的ＲＡＰＤ谱带相似率为９７．０％－９７．８％，与母本相似率为３０．３％－５９．５％，子代中的非亲本谱眩为０－０．０２９，极少母本特异谱带。这一结果说明雄性发育鱼类单倍体的遗传信息主要来自父本，且存在着个体差异，雄核发育子代存在ＤＮＡ变异和母本ＤＮＡ非特异带，但并非其必要条件。

RAPD Analysis of Artificially Induced Androgenesis Haploid of Misgurnus anguillicandatus and Paramisgurnus dabryanus

An overall RAPD analysis of three groups of adrogenesis haploid of Misgurnus anguillicandatus, two groups of androgenesis haploid of Paramisgunus dabryanus and their parents were made by selected 30 primers of radom decamer oligonucleotide. The results showed:the similarity index was 97.0%～97.8% between androgenesis haploid and their male parents; 30.3%～59.5% between androgenesis haploid and their female parents ;the non-parent band ratio of androgenesis haploid was 0～0.029; mother specific band was rarely found in the androgenetic haploid. It was concluded that the major genetic informations of androgenetic hapolid came from their male parents; moreover there were differences among the androgenetic haploid produced by different sire; the non-parents bands and maternal specific bands would be unnecessary for androgenesis haploid.