Purification of rat liver enzymes involved in the oxidation of glyoxylate |
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| Authors: | Hassan Asker David Davies |
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| Institution: | Institut National de la Recherche Agronomique, La Grande Ferrade, 33140 Pont-de-la-Maye, Bordeaux France |
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| Abstract: | The use of Sepharose aminohexyl oxamate for the purification of glycolate oxidase and lactate dehydrogenase is described. The kinetics of both enzymes are reported in relation to their possible roles in the production of oxalate. A model is proposed in which glycolate oxidase in the peroxisomes and lactate dehydrogenase in the cytosol cooperate in the production of oxalate. |
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| Keywords: | Glycolate oxidase Lactate dehydrogenase Glyoxylate metabolism Affinity chromatography (Rat liver) |
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