3′:5′-Cyclic-nucleotide phosphodiesterase in the bovine pituitary gland |
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Authors: | Akio Nagasaka Setsuya Ohkubo Hiroyoshi Hidaka |
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Institution: | 1. Department of Internal Medicine, Fujita-Gakuen University School of Medicine, Toyoake, Aichi 470-11, Japan;2. Department of Pharmacology, Mie University School of Medicine, Tsu, Mie 514, Japan |
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Abstract: | Cyclic-AMP phosphodiesterase activity in the homogenate of the anterior pituitary gland was 2-fold higher than that in the homogenate of the posterior pituitary, whereas cyclic-GMP phosphodiesterase activity was dominant in the posterior homogenate. There were two peaks of cyclic-AMP phosphodiesterase activity with different isoelectric points of 4.3 and 5.2. Fraction I had a molecular weight of 240 000 and a sedimentation coefficient of 6.2 S; fraction II had a molecular weight of 180 000 and a sedimentation coefficient of 3.1 S. Cyclic AMP hydrolytic activity in the supernatant of the posterior lobe corresponded to fraction I in the anterior lobe. Cyclic GMP hydrolytic activity in both the anterior and posterior lobes (activated by Ca2+ / calmodulin) had an isoelecteric point of 5.2, a molecular weight of 240 000 and a sedimentation coefficient of 6.2 S. Cyclic AMP and GMP hydrolytic activities in both the anterior and posterior lobes appeared in fraction I and did not separate when the preparations were mixed before electric focusing or sucrose density gradient procedures. Cyclic AMP hydrolytic activity in fraction II could be separated from cyclic GMP hydrolytic activity. |
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Keywords: | Phosphodiesterase Cyclic nucleotide (Bovine pituitary gland) EGTA |
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