Specific detection of N-acetylglucosamine-containing oligosaccharide chains on ovine submaxillary asialomucin |
| |
Authors: | Dirk H Van den Eijnden Wietske ECM Schiphorst Eric G Berger |
| |
Institution: | 1. Department of Medical Chemistry, Vrije Universiteit, Van der Boechorststraat 7, 100 MC Amsterdam The Netherlands;2. Medizinisch-Chemisches Institut Universität Bern, 3000 Bern 9 Switzerland |
| |
Abstract: | Human milk β-N-acetylglucosaminide β1 → 4-galactosytransferase (EC 2.4.1.38) was used to galactosylate ovine submaxillary asialomucin to saturation. The major 14C]galactosylated product chain was obtained as a reduced oligosaccharide by β-elimination under reducing conditions. Analysis by Bio-Gel filtration and gas-liquid chromatography indicated that this compound was a tetrasaccharide composed of galactose, N-acetylglucosamine and reduced N-acetylgalactosamine in a molar ratio of 2:0.9:0.8. Periodate oxidation studies before and after mild acid hydrolysis in addition to thin-layer chromatography revealed that the most probable structure of the tetrasaccharide is . Thus it appears that Galβ1 → 3(GlcNAcβ1 → 6)GalNAc units occur as minor chains on the asialomucin. The potential interference of these chains in the assay of α-N-acetylgalactosaminylprotein β1 → 3-galactosyltransferase activity using ovine submaxillary asialomucin as an receptor can be counteracted by the addition of N-acetylglucosamine. |
| |
Keywords: | Carbohydrate structure Asialomucin (Ovine submaxillary gland) NeuAc GalNAc GlcNAc Gal galactosel Fuc UDP-Gal uridine diphosphogalactose Galol reduced galactose GalNAcol GlcNAcol reduced glucosamine |
本文献已被 ScienceDirect 等数据库收录! |