Tobacco BY-2 cell-free extracts induce the recovery of microtubule nucleating activity of inactivated mammalian centrosomes |
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| Authors: | Stoppin-Mellet V Peter C Buendia B Karsenti E Lambert A M |
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| Affiliation: | Institut de Biologie Moléculaire des Plantes du CNRS, UPR406, Université Louis Pasteur, 12 rue du Général Zimmer, 67084, Strasbourg Cedex, France. virginie.stoppin-mellet@ibmp-ulp.u-strasbg.fr |
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| Abstract: | The structure and the molecular composition of the microtubule-organizing centers in acentriolar higher plant cells remain unknown. We developed an in vitro complementation assay where tobacco BY-2 extracts can restore the microtubule-nucleating activity of urea-inactivated mammalian centrosomes. Our results provide first evidence that soluble microtubule-nucleating factors are present in the plant cytosolic fraction. The implication for microtubule nucleation in higher plants is discussed. |
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