Glutamine limited fed-batch culture reduces ammonium ion production in animal cells

Summary In a glutamine limited fed-batch culture of the murine myeloma cell Sp 2/0-Ag 14 the production of ammonium ions was reduced to half of that produced in an ordinary batch culture. Other parameters like , DOT, length of stationary phase, the quotients lactate/glucose, ammonium/glutamine, and alanine/glutamine were also influenced by the feeding technique.