EFFICACY OF AN IMMUNO-MAGNTIC SEPARATION SYSTEM FOR RECOVERING CRYPTOSPORIDIUM OOCYSTS FROM SOILS |
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| Authors: | WYNDI McELROY ELISSETTE CABELLO SURESH D PILLAI |
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| Institution: | Poultry Science Department and Institute of Food Science and Engineering Texas A&M University College Station, Texas 77843–2472 |
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| Abstract: | Studies were conducted to determine the efficacy of the commercially available immuno-magnetic system by DynalTM to recover C. parvum oocysts from silty clay, sandy clay loam and clay soils. Each soil type was spiked with known numbers of oocysts and their recovery using percoll-sucrose gradient centrifugation in combination with immuno-magnetic separation system was evaluated. The recoveries varied significantly. The silty clay loam soil had the highest recovery ranging between 91% and 26%, while the sandy clay loam had the lowest recovery ranging between 30% and 2%. The results indicate that though the DynalTM IMS system is capable of recovering oocysts from soils, the recovery efficiencies can vary significantly. |
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