Major acute-phase alpha(1)protein in the rat: structure, molecular cloning, and regulation of mRNA levels |
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| Authors: | T Cole A Inglis M Nagashima G Schreiber |
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| Affiliation: | 1. The Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, 3052, Australia;1. Centre of Advanced Study, Department of Pharmacognosy, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, India;2. Pharmacognosy and Phytotherapy Research Laboratory, Division of Pharmacognosy, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India;1. Biomedical System and Technology Group, Korea Institute of Industrial Technology, Cheonan, 31056, South Korea;2. Samsung Research, Samsung Electronics, Seoul R&D Campus, Seoul, 06765, South Korea;3. Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, 37673, South Korea;1. Department of Industrial and Systems Engineering, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region of China;2. Department of Industrial and Systems Engineering, Research Institute for Advanced Manufacturing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Special Administrative Region of China;3. Department of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China;4. School of Materials Science and Engineering, Guangdong Engineering Centre for Petrochemical Energy Conservation, Sun Yat-sen University, Guangzhou, 510275, China;5. Department of Business Administration, University of Verona, Verona, 37129, Italy;1. Institute of New Energy for Vehicles, Shanghai Key Laboratory for R&D and Application of Metallic Functional Materials, School of Materials Science and Engineering, Tongji University, Shanghai 201804, PR China;2. State Key Laboratory of Material Processing and Die & Mould Technology, School of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, PR China |
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| Abstract: | Rat major acute-phase alpha(1)protein (MAP) was characterized by determining its secondary structure, ligand binding and partial amino acid sequence. A cDNA clone expressing MAP and coding for the entire mature protein was isolated from a cDNA library in E. coli prepared from rat liver mRNA. By hybridization to nick translated cDNA, mRNA for MAP was found only in liver, where it increased 17-fold during acute inflammation. Constant proportions of rates of leucine incorporation into MAP over mRNA levels in liver indicated that the regulation of the synthesis of MAP is due to a change in the rate of synthesis and/or the stability of mRNA for MAP, but not the rate of its translation. |
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