Crystallization of the A alpha subunit of protein phosphatase 2A. |
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| Authors: | M. O. Skidmore M. R. Sawaya S. Parkin B. Rupp H. Hope S. J. Everse G. Walter |
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| Affiliation: | Department of Pathology, University of California at San Diego, La Jolla 92093-0612, USA. |
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| Abstract: | The A alpha subunit of human protein phosphatase 2A forms crystals in space group P2(1) with cell dimensions a = 104.0, b = 174.9, c = 168.2 A, and beta angle = 90.2 degrees. At cryogenic temperatures, the crystals diffracted to a resolution limit of approximately 3.0 A. Based on the unit cell dimensions and a calculated molecular mass of 65,277 Da, the Matthews coefficient suggests eight molecules per asymmetric unit. Two native data sets were collected to a nominal resolution of 3.0 A and merged to provide a set that is 93% complete, with Rsym of 9.9%. |
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