The maintenance affinities of KLF-1 proximal F merogenotes inEscherichia coli |
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Institution: | (1) Department of Bacterial Genetics, Institute of Microbiology, Czechoslovak Academy of Sciences, Budějovická 1083, 142 20 Prague 4, Czechoslovakia |
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Abstract: | Summary In conjugation with donor strains carrying proximal F merogenotes of KLF-1 type about 100-fold lower frequency ofLeu
+ orLac
+ recombinants was found. The determination of the level of β-galactosidase synthesis during the initial period of mating indicated
that the transfer process of plasmid DNA was not impaired. Among the recombinants selected a large fraction have not expressed
the plasmic fertility functions. This phenomenon was found to be replicon specific and was observed only with proximal F merogenotes
but not with classical F'lac and F'ORF-1 elements or RI-19 plasmid. The expression of KLF-1 plasmid functions in the cell seems to be affected by a chromosomal
gene of the proximal F merogenote closely linked toleu marker. |
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