Production of antifungal metabolites by the ectomycorrhizal fungusPisolithus tinctorius strain SMF |
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Authors: | Hyung-Won Suh Don L Crawford Roger A Korus Kalidas Shetty |
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Institution: | (1) Department of Bacteriology and Biochemistry, Institute for Molecular and Agricultural Genetic Engineering, University of Idaho, 83843 Moscow, ID, USA;(2) Department of Chemical Engineering, Institute for Molecular and Agricultural Genetic Engineering, University of Idaho, 83843 Moscow, ID, USA |
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Abstract: | Summary An ectomycorrhizal fungus,Pisolithus tinctorius strain SMF, isolated from a basidiocarp removed from the roots of a recently fallen old growth fir in the Smoky Mountains of Tennessee, was characterized for its in vitro production of antifungal metabolites. On solid mediumP. tinctorius SMF strongly inhibited growth of strains ofFusarium solani, Geotrichum candidum, Phanerochaete chrysosporium, andVerticillium dahliae, all species known to be plant pathogens. Evidence from paired colony growth inhibition studies on agar plates indicated that production of antifungal agents byP. tinctorius SMF may be enhanced by close physical contact with other fungi. The antifungal activity ofP. tinctorius SMF was much greater than that of several culture collection strains ofP. tinctorius. The culture collection strains either showed no or very limited activity. The antifungal activity was associated with an apparently inducible metabolism ofP. tinctorius SMF and with the production of darkly colored water soluble phenolic metabolites. Small scale fermentation studies showed that the phenolics are readily producible by submerged culture fermentation. This is the first report of submerged culture production of antifungal metabolites by an ectomycorrhizal fungus. |
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Keywords: | Ectomycorrhizal Pisolithus Antifungal Phenolic Fermentation |
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