Primary structure and biological activity of bradykinin potentiating peptides fromBothrops insularis snake venom |
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Authors: | Adélia C. O. Cintra Carlos A. Vieira José R. Giglio |
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Affiliation: | (1) Departamento de Bioquímica, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, 14.049 Ribeirão Preto-SP, Brasil |
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Abstract: | Several bradykinin potentiating peptides (BPPs) were isolated from the venom of the Brazilian arboricole snake Bothrops insularis by gel filtration on Sephadex G-150-120, followed by sequencial high-voltage paper electrophoreses atpH 3.5, 6.5, and 2.1. The BPPs were assayed by their ability to potentiate the contractile activity, on the isolated guinea pig ileum, and the hypotensive activity, on anesthetized rats, of bradykinin. Eight BPPs, containing 3–13 amino acid residues, were sequenced and their primary structures were shown to have a marked degree of homology with those of several BPPs from other venoms. |
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Keywords: | Bradykinin potentiating peptides Bothrops insularis venom sequencing homology |
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