Two plasmid-determined restriction and modification systems in Streptococcus lactis |
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| Authors: | Alain Chopin Marie-Christine Chopin Agns Moillo-Batt Philippe Langella |
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| Institution: | 1. Institut National de la Recherche Agronomique, Laboratoire de Recherches de Technologie Laitière, 35042, Rennes, France;2. Institut National de la Santé et de la Recherche Médicale, Laboratoire de Biochimie Médicale B, Faculté de Médecine, 35043, Rennes, France |
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| Abstract: | Two restriction and modification systems were found in Streptococcus lactis strain IL594 which was found to contain 9 plasmids designated pIL1 to pIL9. On the basis of protoplast-induced curing experiments, we showed that a restriction and modification system was related to the presence of pIL6 or pIL7. The pIL6-determined restriction and modification system was confirmed by cotransfer of the plasmid and of the restriction and modification system to a plasmid-free, nonrestricting, and nonmodifying derivative of S. lactis IL594. |
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