棉铃虫不同寄主种群遗传分化的RAPD分析

应用RAPD技术对生活于辣椒，烟草和番茄3种不同寄主上的棉铃虫（Helicover pa armigera)种群，并以烟草烟青虫（H.assulta)为外群进行了种群遗传分化的研究，研究结果表明：4个处理内个体间最大的遗传距离均小于处理间最小的遗传距离，烟青虫的遗传距离为0．177－0．346，棉铃虫的辣椒种群为0．289－0．404，烟草种群为0．396－0．505，番茄种群为0．329－0．382，采用UPDGA法进行聚类，并构建系统发育树。各处理成聚的遗传距离为：烟青虫是0．334，棉铃虫的辣椒种群是0．372，烟草种群是0．463，番茄种群是0．360。各处理均首先成聚，棉铃虫与烟青虫成聚的遗传距离（0．703），稍大于棉铃虫各种群的成聚值（0．639）。上述结果表明，棉铃虫不同寄主种群已经存在明显的遗传分化。

The RAPD analysis of genetic differentiation for the different host populations of Helicoverpa armiger

The genetic differentiation of Helicoverpa armigera populations on chillier, tobacco and tomato were studied by RAPD, with H. assulta as outgroup. The result showed that the most genetic distance in the population was less than the least genetic distance between the different populations. In the populations, the genetic distance of H. assulta was 0.177-0.346, and the chillier population of Helicoverpa armigera was 0.289-0.414, the tobacco population 0.396-0.505, the tomato population 0.329-0.382. The phylogenic tree was formed by UPDGA. The genetic distance of clustering populations was 0.334 for H. assulta , 0.372 for the cillier population, 0.463 for the tobacco population and 0.36 for the tomato population of Helicoverpa armigera . Each population was clustered at first, and the genetic distance (0.703) of clustering for the two species, Helicoverpa armigera and H.assulta , was a little more than the all populations of H. armigera (0.639). The above result indicated that the different host populations of H.armigera existed distinctly genetic differentiation .