Direct triazine herbicide detection using a self-assembled photosynthetic reaction center from purple bacterium |
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| Authors: | Chikashi Nakamura Miki Hasegawa Kazumi Shimada Makoto Shirai Jun Miyake |
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| Affiliation: | (1) National Institute for Advanced Interdisciplinary Research, AIST, MITI 1-1-4 Higashi, 305-8562 Tsukuba, Ibaraki, Japan;(2) Division of Biotechnology School of Agriculture, Ibaraki University, 300-0331 Ami, Ibaraki, Japan |
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| Abstract: | In this study, a direct detection system for triazine derivative herbicides was developed using the photosynthetic reaction center (RC) from the purple bacterium,Rhodobacter sphaeroides, and surface plasmon resonance (SPR) apparatus. The histidine-tagged RCs were immobilized on an SPR gold chip using nickel-nitrilotriacetic acid groups as a binder for one of the triazine herbicide, atrazine. The SPR responses were proportional to the sample concentrations of atrazine in the range 0.1–1 μg/mL. The sensitivity of the direct detection of atrazine using the RC-assembled sensor chip was higher than that using the antibody-immobilized chip. The other types of herbicides, DCMU or MCPP, were not detected with such high sensitivity. The results indicated the high binding selectivity of the RC complex. |
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| Keywords: | photosynthetic reaction center herbicide triazine derivative surface plasmon resonance biosensor affinity binding |
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