Inulin hydrolysis by the Debaryomyces phaffii inulinase immobilized on Deae cellulose |
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| Authors: | Joseph Pierre Guiraud Sylvie Demeulle P. Galzy |
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| Affiliation: | (1) Sciences et Technologies des Industries Alimentaires USTL, 34060 Montpellier, France;(2) Laboratoire de Génétique et Microbiologie ENSAM/INRA, 34060 Montpellier, France |
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| Abstract: | Debaryomyces phaffii inulinase was immobilized by adsorption on DEAE-cellulose. The immobilization caused a drop in optimum pH, a slight increase in optimum temperature and an important increase in the thermal stability of the system. The activity yield of immobilized inulinase was 75%.A continuous reactor operation was carried out. The utilization of this system permited the production of fructose syrup from inulin. |
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