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Inulin hydrolysis by the Debaryomyces phaffii inulinase immobilized on Deae cellulose
Authors:Joseph Pierre Guiraud  Sylvie Demeulle  P Galzy
Institution:(1) Sciences et Technologies des Industries Alimentaires USTL, 34060 Montpellier, France;(2) Laboratoire de Génétique et Microbiologie ENSAM/INRA, 34060 Montpellier, France
Abstract:Debaryomyces phaffii inulinase was immobilized by adsorption on DEAE-cellulose. The immobilization caused a drop in optimum pH, a slight increase in optimum temperature and an important increase in the thermal stability of the system. The activity yield of immobilized inulinase was 75%.A continuous reactor operation was carried out. The utilization of this system permited the production of fructose syrup from inulin.
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