Brain Tubulin Microheterogeneity in the Mouse During Development and Aging |
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| Authors: | Kern von,Hungen,Rustum C. Chin,&dagger ,Claude F. Baxter&Dagger |
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| Affiliation: | Neurochemistry, Brain Biochemistry Laboratories, Veterans Administration Medical Center, Sepulveda, California;Neurochemistry, Departments of Biological Chemistry, Los Angeles, California U.S.A.;Neurochemistry, Psychiatry, UCLA Center for the Health Sciences, Los Angeles, California U.S.A. |
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| Abstract: | Abstract: Mouse brain tubulin was analyzed on isoelectric focusing gels. High-resolution gels utilizing Bio-Rad ampholytes (pH 4-6) revealed 5-6 bands in the region corresponding to the α-subunit of tubulin and 10 or more for the β-subunit. The same general banding pattern was observed regardless of the method of preparation of the tubulin. Two species prominent in the brains of immature mice, α6 and β2, virtually disappeared during maturation, while species β6 to β10 appeared. No significant changes from the mature pattern were seen during aging (examined at 12, 23, and 30 months of age). |
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| Keywords: | Brain tubulin Tubulin microheterogeneity Isoelectric focusing Aging Mouse brain |
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