| 两种缘毛类纤毛虫胞质Hsp70基因序列的克隆及其系统发育分析 |
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| 引用本文： | 傅诚杰,缪炜,沈韫芬,万明亮.两种缘毛类纤毛虫胞质Hsp70基因序列的克隆及其系统发育分析[J].动物学研究,2004,25(4):298-303. |
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| 作者姓名： | 傅诚杰 缪炜 沈韫芬 万明亮 |
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| 作者单位： | 1. 中国科学院东湖湖泊生态系统试验站,湖北,武汉,430072;中国科学院水生生物研究所,湖北,武汉,430072
2. 中国科学院水生生物研究所,湖北,武汉,430072 |
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| 基金项目： | 中国科学院创新工程基金资助项目 ( 2 2 0 2 0 7)，东湖湖泊生态系统试验站开放基金资助项目 ( 2 0 0 40 5 ) |
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| 摘 要： | 克隆得到2种缘毛类纤毛虫——钟形钟虫(Vorticella campanula)和螅状独缩虫(Carchesium polypinum)的胞质Hsp70基因部分序列，长度均为438bp，编码146个氨基酸。以细菌为外类群，利用最大似然法和邻接法构建包括其他5种纤毛虫在内的共26个物种的Hsp70基因氨基酸序列系统发育树，其拓扑结构显示：V．campanula和C．polypinum聚在一起，并与另2种寡膜纲的嗜热四膜虫(Tetrahymena thermophila)及草履虫(Paramecium tetraurelia)聚为姊妹枝，提示了缘毛类纤毛虫为单系，且隶属于寡膜纲的系统发育地位。
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| 关 键 词： | 缘毛类纤毛虫 胞质 Hsp70基因 克隆 系统发育 |
| 文章编号： | 0254-5853(2004)04-0298-06 |
Phylogenetic Analysis of Peritrichs Inferred from Cytosolic Hsp70 Gene Partial Sequences |
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| FU Cheng-jie.Phylogenetic Analysis of Peritrichs Inferred from Cytosolic Hsp70 Gene Partial Sequences[J].Zoological Research,2004,25(4):298-303. |
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| Authors: | FU Cheng-jie |
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| Institution: | 1.Donghu Experimental Station of Lake Ecosystems,the Chinese Academy of Sciences,Wuhan 430072 2.Institute of Hydrobiology,the Chinese Academy of Sciences,Wuhan 430072,China |
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| Abstract: | Cytosolic Hsp70 genes of two peritrichous ciliates (Vorticella campanula & Carchesium polypinum) were partially sequenced and the lengths are 438 bp,both encoding 146 amino acids.Using bacteria as the outgroup,the phylogenetic trees were constructed based on cytosolic Hsp70 amino acid sequences by maximum-likelihood (ML) and neighbor-joining (NJ) methods.The topology that V.campanula clustered with C.polypinum,and then formed a sister clade with another two species in Oligohymenophorea (Tetrahymena thermophila & Paramecium tetraurelia),suggested the monophyly of the peritrichs and their phylogenetic placement in the class Oligohymenophorea. |
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| Keywords: | Peritrichs Cytosolic Hsp70 gene Phylogeny |
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