联合应用大鼠血管内皮细胞和胰岛培养对胰岛功能的影响

目的 探讨通过体外共培养胰岛和血管内皮细胞能否改善胰岛的功能.方法 SD 大鼠分离纯化出胰岛细胞,分为两组：A 组胰岛单纯培养组,B 组胰岛和内皮细胞共培养组.从大鼠的胸主动脉分离纯化出血管内皮细胞,胰岛分离纯化后通过AO/PI 染色和胰岛素释放实验来判断两组胰岛的活性.结果 共培养组胰岛在7 d 内维持正常的形态,90﹪的胰岛通过AO/PI 染色显示良好的活性;胰岛素释放实验显示第7 天（2.21 ± 0.21）和第14 天（2.53 ± 0.21）共培养组和单纯培养组（1.94 ± 0.15,1.71 ± 0.19）刺激指数差异有统计学意义（P 〈 0.05）.结论 应用大鼠血管内皮细胞和胰岛共培养能够改善胰岛的存活及分泌功能.

Effect of endothelial cell coculture on islet function

Objective To investigate whether the function of isolated islets can be retained by coculture with thoracic aorta endothelial cells （AEC） in vitro. Methods The islets were isolated from SD rats in this study. In group A： the islets were cultured alone; in group B, the islets were cultured with ECs.The ECs were isolated from thoracic aorta. The viability of the isolated islets was assessed by AO/PI double staining. Islet function was assessed by insulin release assay. Results Islets in group B exhibited normal morphology, with greater than 90 % staining positive for AO/PI at 7 days. Insulin release assay showed that there was a significantly higher simulation index （SI） in group B （2.21± 0.21, 2.53± 0.21） as compared with group A（1.94 ± 0.15, 1.71 ± 0.19） at 7 days and 14 days （P〈 0.05 ）. Conclusion This study suggests that co-culture of freshly isolated rat islets with ECs can improve islet survival and function in vitro.