Effects of vascular endothelial growth factor B on proliferation and migration in EA.Hy926 cells |
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Authors: | Guang-hong Zhang Rui Qin Shui-hua Zhang He Zhu |
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Affiliation: | 1. Department of Ophthalmology, The No.474 Hospital of Chinese People’s Liberation Army, Urumchi, 830011, China 2. Department of Ophthalmology, The People’s Hospital of Bozhou, Bozhou, 236804, China 3. Department of Ophthalmology, The Second Clinical College, Harbin Medical University, Harbin, 150086, China 4. Department of Anesthesiology, The Second Clinical College, Harbin Medical University, Harbin, 150086, China
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Abstract: | Vascular endothelial growth factor B (VEGF-B) was reported to be angiogenic, and it was considered as a neuroprotective agent in mouse retinal ganglion cells following optic nerve crush. Thus, it was necessary to investigate whether VEGF-B contributes to the process of retinal and choroidal neovascularization. We aimed to investigate the effects of VEGF-B on proliferation and migration in EA.Hy926 cells. The proliferation of cells was analyzed by cell counting kit 8 assay, and the migration of cells was evaluated by a modified Boyden chamber assay. The levels of phospho-ERK1/2 (P-ERK1/2), ERK1/2, phospho-p38 and p38 were detected by western blotting. The results showed that VEGF-B induced proliferation and migration of EA.Hy926 cells (P < 0.01 and P < 0.05, respectively), and ERK1/2 and p38 phosphorylation were significantly activated. Our study suggested that VEGF-B was an angiogenesis factor in vitro and that ERK1/2 and p38-related signaling pathways were involved in these VEGF-B activities. |
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