Correlation of the guanosine exchangeable and nonexchangeable base protons in 13C-/15N-labeled RNA with an HNC-TOCSY-CH experiment |
| |
| Authors: | Jean-Pierre Simorre Grant R. Zimmermann Luciano Mueller Arthur Pardi |
| |
| Affiliation: | (1) Department of Chemistry and Biochemistry, University of Colorado at Boulder, 80309-0215 Boulder, CO, USA;(2) Bristol-Myers Squibb Pharmaceutical Research Institute, P.O. Box 4000, 08543-4000 Princeton, NJ, USA |
| |
| Abstract: | Summary A triple resonance HNC-TOCSY-CH experiment is described for correlating the guanosine imino proton and H8 resonances in 13C-/15N-labeled RNAs. Sequential assignment of the exchangeable imino protons in Watson-Crick base pairs is generally made independently of the assignment of the nonexchangeable base protons. This H(NC)-TOCSY-(C)H experiment makes it possible to unambiguously link the assignment of the guanosine H8 resonances with sequential assignment of the guanosine imino proton resonances. 2D H(NC)-TOCSY-(C)H spectra are presented for two isotopically labeled RNAs, a 30-nucleotide lead-dependent ribozyme known as the leadzyme, and a 48-nucleotide hammerhead ribozyme-RNA substrate complex. The results obtained on these two RNAs demonstrate that this HNC-TOCSY-CH experiment is an important tool for resonance assignment of isotopically labeled RNAs. |
| |
| Keywords: | RNA Nucleic acids Isotope label Guanosine Purine |
| 本文献已被 SpringerLink 等数据库收录！ |
|
