Antifungal effect with apoptotic mechanism(s) of Styraxjaponoside C |
| |
| Authors: | Cana Park Dong Gun Lee |
| |
| Institution: | a School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Puk-ku, Daegu 702-701, Republic of Korea
b College of Pharmacy, Chosun University, Gwangju 501-759, Republic of Korea |
| |
| Abstract: | The antifungal effects and mechanisms of Styraxjaponoside C were investigated. Styraxjaponoside C was active against several human pathogens, including Candida albicans. Styraxjaponoside C induced a series of cellular changes characteristic of apoptosis in C. albicans, including increased reactive oxygen species (ROS) production, measured by DHR-123 staining; phosphatidylserine externalization, visualized by Annexin V staining; DNA fragmentation, as seen by TUNEL; and plasma membrane depolarization, observed by DiBAC4(3) staining. The plasma membrane depolarization is likely to be associated with production of ROS. The current study suggests that Styraxjaponoside C exerts an antifungal effect by promoting apoptosis. |
| |
| Keywords: | MTT (3-(4 5-dimethyl-2-thiazolyl)-2 5-diphenyl-2H-tetrazolium bromide) DiBAC4(3) bis-(1 3-dibutylbarbituric acid) trimethine oxonol CCCP carbonyl cyanide m-chlorophenylhydrazone PI propidium iodide TUNEL terminal deoxynucleotidyltransferase dUTP nick end labeling ROS reactive oxygen species DHR dihydrorhodamine NAC l-cysteine" target="_blank">N-Acetyl-l-cysteine |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
