Isolation and chromosomal localization of a germ line-specific highly repetitive DNA family in Acricotopus lucidus (Diptera, Chironomidae) |
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Authors: | Wolfgang Staiber Irmgard Wech Anette Preiss |
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Institution: | (1) Institute of Genetics, University of Hohenheim, D-70593 Stuttgart, Germany, DE |
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Abstract: | . In the chironomid Acricotopus lucidus, parts of the genome, the germ line-limited chromosomes, are eliminated from the future soma cells during early cleavage
divisions. A highly repetitive, germ line-specific DNA sequence family was isolated, cloned and sequenced. The monomers of
the tandemly repeated sequences range in size from 175 to 184 bp. Analysis of sequence variation allowed the further classification
of the germ line-restricted repetitive DNA into two related subfamilies, A and B. Fluorescence in situ hybridization to gonial
metaphases demonstrated that the sequence family is highly specific for the paracentromeric heterochromatin of the germ line-limited
chromosomes. Restriction analysis of genomic soma DNA of A. lucidus revealed another tandem repetitive DNA sequence family with monomers of about 175 bp in length. These DNA elements are found
only in the centromeric regions of all soma chromosomes and one exceptional germ line-limited chromosome by in situ hybridization
to polytene soma chromosomes and gonial metaphase chromosomes. The sequences described here may be involved in recognition,
distinction and behavior of soma and germ line-limited chromosomes during the complex chromosome cycle in A. lucidus and may be useful for the genetic and cytological analysis of the processes of elimination of the germ line-limited chromosomes
in the soma and germ line.
Received: 12 April 1997; in revised form 26 June 1997 / Accepted: 29 June 1997 |
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