Somatic embryogenesis in the medicinal legume <Emphasis Type="Italic">Desmodium motorium</Emphasis> (Houtt.) Merr. |
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Authors: | B Chitra Devi V Narmathabai |
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Institution: | (1) Department of Biotechnology, Karpagam University, Coimbatore, Tamil Nadu, 641 021, India;(2) Department of Botany, Bharathiar University, Coimbatore, 641 046, India |
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Abstract: | An efficient protocol was established for regeneration of Desmodium motorium via somatic embryogenesis. Embryogenic calli were induced from cotyledon segments (6 mm, 16 days old) lacking embryo axis,
excised from seedlings grown in vitro on Murashige and Skoog (MS) medium supplemented with indole-3-acetic acid (IAA) (2.9 μM)
in combination with 6-benzyladenine (BA) (4.44 and 8.88 μM). Differentiation of embryogenic calli into globular and heart-shaped
somatic embryos was achieved on transfer to hormone-free MS medium. When incubated for 4 days on MS medium supplemented with
BA (8.88 μM), 95% of the globular and heart-shaped somatic embryos matured into torpedo and cotyledonary stages with minimum
(10%) abnormalities. Modified MS basal medium without hormones and containing half-strength macronutrients and 0.88 M sucrose
was suitable for germination of mature somatic embryos. Regenerated plantlets were successfully transferred to earthen pots
with survival rate of 50%. Secondary embryogenesis was observed when pre-existing somatic embryos at globular and heart-shaped
stages were cultured on MS medium supplemented with various concentrations of BA, adenine sulphate (AdS) and abscisic acid
(ABA) individually. |
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