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Synthesis and storage of a neurohormone in insect brains in vitro |
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| Authors: | E.P. Marks G.M. Holman T.K. Borg |
| Affiliation: | 1. Metabolism and Radiation Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Fargo, North Dakota 58102 USA;2. Faculty, Division of Natural Sciences, North Dakota State University, Fargo, North Dakota 58102 USA |
| Abstract: | The build-up of neurosecretory material in the median neurosecretory cells and fibre tracts of cultured cockroach brains was demonstrated by staining and bioassay. Examination of the cultured brains by electron microscopy showed active production of neurosecretory granules after 3 days in vitro. The close correlation of the results obtained by these different methods of assay leaves little doubt that a neurohormone is being synthesized and stored. |
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