首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Thermophilic enrichment of microbial communities in the presence of the ionic liquid 1‐ethyl‐3‐methylimidazolium acetate
Authors:AP Reddy  CW Simmons  J Claypool  L Jabusch  H Burd  MZ Hadi  BA Simmons  SW Singer  JS VanderGheynst
Institution:1. Joint BioEnergy Institute, , Emeryville, CA, USA;2. Biological and Agricultural Engineering, University of California‐Davis, , Davis, CA, USA;3. Earth Sciences Division, Lawrence Berkeley National Laboratory, , Berkeley, CA, USA;4. Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, , Livermore, CA, USA;5. Department of Biomass Science and Conversion Technology, Sandia National Laboratories, , Livermore, CA, USA
Abstract:

Aims

The aim of the study was to develop an approach to enrich ionic liquid tolerant micro‐organisms that efficiently decompose lignocellulose in a thermophilic and high‐solids environment.

Methods and Results

High‐solids incubations were conducted, using compost as an inoculum source, to enrich for thermophilic communities that decompose switchgrass in the presence of the ionic liquid 1‐ethyl‐3‐methylimidazolium acetate (C2mim]OAc]). Ionic liquid levels were increased from 0 to 6% on a total weight basis incrementally. Successful enrichment of a community that decomposed lignocellulose at 55°C in the presence of 6% C2mim]OAc] was achieved, when the C2mim]OAc] level was increased stepwise from 2% to 4% to 5% to 6%. Pyrosequencing results revealed a shift in the community and a sharp decrease in richness, when thermophilic conditions were applied.

Conclusions

A community tolerant to a thermophilic, high‐solids environment containing 6% C2mim]OAc] was enriched from compost. Gradually increasing C2mim]OAc] concentrations allowed the community to adapt to C2mim]OAc].

Significance and Impact of the Study

A successful approach to enrich communities that decompose lignocellulose under thermophilic high‐solids conditions in the presence of elevated levels of C2mim]OAc] has been developed. Communities yielded from this approach will provide resources for the discovery of enzymes and metabolic pathways relevant to biomass pretreatment and fuel production.
Keywords:1‐ethyl‐3‐methylimidazolium acetate  compost  ionic liquid tolerance  microbial community
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号