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Enzymes catalyzed esterification of N-protected amino acids with secondary alcohols |
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| Authors: | I.-L. Shih L.-C. Chiu C.T. Lai W.-C. Liaw D.-F. Tai |
| Affiliation: | (1) Biochemical Engineering Center, Da-Yeh Institute of Technology, Chang Hwa, Taiwan;(2) Department of Chemical Engineering, National Yunlin Institute of Technology, Yunlin, Taiwan;(3) Department of Chemistry, National Dong-Hwa University, Hualien, Taiwan ROC |
| Abstract: | Secondary alcohols were converted to their corresponding esters with N-Cbz-L-amino acids by Celite-immobilized protease or lipase in organic solvents at pH 7.5. The esterification of 2-butanol and 2-phenylethanol were achieved up to 71% yields. The optimal reaction condition for Aspergillus oryzae protease (AOP) catalyzed synthesis of N-Cbz-L-aspartyl- sec-butyl ester (97.6 de) was at pH 7.5 and a duration of 96 hours. |
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