| 转Bt基因玉米对根际土壤细菌群落结构的影响 |
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| 引用本文： | 邹雨坤,张静妮,杨殿林,陈秀蓉. 转Bt基因玉米对根际土壤细菌群落结构的影响[J]. 生态学杂志, 2011, 30(1): 98-105 |
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| 作者姓名： | 邹雨坤 张静妮 杨殿林 陈秀蓉 |
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| 作者单位： | 1. 农业部环境保护科研监测所农业部转基因生物生态环境安全监督检验测试中心,天津300191;中国农业科学院武清转基因生物农田生态系统影响野外科学观测试验站,天津300191
2. 甘肃农业大学草业学院草业生态系统教育部重点实验室,中-美草地畜牧业可持续发展研究中心,兰州730070 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金(农业部环境保护科研监测所)项目(2009-zjn-10); 转基因生物新品种培育重大专项(2008ZX08012-004); 国家自然科学基金项目(30770367)资助 |
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| 摘 要： | 利用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术及扩增产物序列分析方法研究了转Bt基因玉米对根际土壤细菌群落结构及系统发育的影响.结果表明:转基因玉米与其非转基因亲本根际共有的土壤细菌分别隶属于变形菌门、疣微菌门、放线菌门、拟杆菌门、厚壁菌门、芽单胞菌门和酸杆菌门.其中,变形菌门为主要优势类群,占43.5%...
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| 关 键 词： | 转基因玉米 细菌 根际土壤 变性梯度凝胶电泳 |
Effects of transgenic Bt maize an bacterial community structure in rhizosphere soil |
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| ZOU Yu-kun,ZHANG Jing-ni,YANG Dian-lin,CHEN Xiu-rong. Effects of transgenic Bt maize an bacterial community structure in rhizosphere soil[J]. Chinese Journal of Ecology, 2011, 30(1): 98-105 |
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| Authors: | ZOU Yu-kun ZHANG Jing-ni YANG Dian-lin CHEN Xiu-rong |
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| Affiliation: | ZOU Yu-kun1,2,ZHANG Jing-ni1,YANG Dian-lin1,CHEN Xiu-rong3(1Agro-Environmental Protection Institute,Ministry of Agriculture,Eco-safety Supervision,Inspection & Testing Center of Genetically Modified Organisms,Tianjin 300191,China,2Wuqing Field Observation Experiment Station for the Impact of Genetically Modified Organisms on Agricultural Ecosystem of Chinese Academy Agricultural Sciences,3Pratacultural College,Gansu Agricultural University,Key Laboratory of... |
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| Abstract: | Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and sequence analysis were employed to assess the effects of transgenic Bt maize on the bacterial community structure and phylogeny in rhizosphere soil. In the rhizosphere soils of transgenic maize and its non-transgenic parents,most sequences were characterized by the members of Proteobacteria,Verrucomicrobia,Actinobacteria,Bacteroidetes,Firmicutes,Gemmatimonadetes,and Acidobacteria,among which,Proteobacteria was the dominant grou... |
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| Keywords: | transgenic Bt maize bacteria rhizosphere soil denaturing gradient gel electrophoresis (DGGE). |
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