| RISA、DGGE和2D-PAGE技术对大豆根际土壤细菌多样性分析的比较 |
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| 引用本文： | 李刚,赵建宁,文都日乐,杨殿林. RISA、DGGE和2D-PAGE技术对大豆根际土壤细菌多样性分析的比较[J]. 生态学杂志, 2011, 30(1): 87-92 |
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| 作者姓名： | 李刚 赵建宁 文都日乐 杨殿林 |
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| 作者单位： | 农业部环境保护科研监测所,农业部转基因生物生态环境安全监督检验测试中心,天津,300191 |
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| 基金项目： | 转基因生物新品种培育重大专项(2008ZX08012-04); 中央级公益性科研院所基本科研业务费专项资金(2008-LG-04)资助 |
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| 摘 要： | 采用基于PCR扩增的核糖体间隔区分析(RISA)、变性梯度凝胶电泳(DGGE)和双向电泳(2D-PAGE)3种分子生态学技术对大豆根际土壤细菌多样性比对分析.结果表明:2D-PAGE技术得到的土壤细菌多样性(基因点)最丰富,其次为DGGE技术(基因片段),RISA技术(基因片段)最低.RISA技术得到的条带数最少,但结...
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| 关 键 词： | RISA DGGE 2D-PAGE 细菌多样性 土壤微生物 |
Bacterial diversity in rhizosphere soil of soybean: A comparison of RISA, DGGE, and 2D-PAGE techniques |
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| LI Gang,ZHAO Jian-ning,WEN DU Ri-le,YANG Dian-lin. Bacterial diversity in rhizosphere soil of soybean: A comparison of RISA, DGGE, and 2D-PAGE techniques[J]. Chinese Journal of Ecology, 2011, 30(1): 87-92 |
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| Authors: | LI Gang ZHAO Jian-ning WEN DU Ri-le YANG Dian-lin |
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| Affiliation: | LI Gang,ZHAO Jian-ning,WEN DU Ri-le,YANG Dian-lin(Ecological Environment Safety Supervision,Inspection and Testing Center of GMOs,Agro-Environmental Protection Institute,Ministry of Agriculture,Tianjin 300191,China) |
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| Abstract: | The PCR-based RISA,DGGE,and 2D-PAGE were adopted to analyze the bacterial diversity in rhizosphere soil of soybean. Most abundant soil bacterial diversity was acquired by 2D-PAGE (gene spots),followed by DGGE (gene bands),and by RISA (gene bands). The RISA gave the fewest bands,but the result was more stable and the experimental operation was more easy; DGGE obtained more bands and showed good precision,but had more error sources; while 2D-PAGE as a novel soil microbial research method could overcome the lo... |
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| Keywords: | RISA DGGE 2D-PAGE bacterial diversity soil microbe. |
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