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Metabolomic analysis of amino acid metabolism in colitic rats supplemented with lactosucrose |
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| Authors: | Zheng Ruan Yinfei Lv Xiaofang Fu Qinghua He Zeyuan Deng Wenqun Liu Yu Yingli Xiaosong Wu Guoyao Wu Xin Wu Yulong Yin |
| Affiliation: | 1. State Key Laboratory of Food Science and Technology, College of Life Science and Food Engineering, Nanchang University, Rm 325, 235 Nanjing Street, Nanchang, 330047, China 2. Analytic Center for Hubei Entry-exit Inspection and Quarantine Bureau, Wuhan, 430031, China 3. Department of Food Science and Engineering, College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen, 518060, China 4. College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, China 5. State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China 6. Department of Animal Science, Faculty of Nutrition, Texas A&M University, College Station, TX, 77843-2471, USA 7. Hunan Engineering and Research Center of Animal and Poultry Science, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, China |
| Abstract: | Intestinal inflammation causes metabolic disorders. The purpose of this study was to determine the effect of dietary supplementation with lactosucrose (LS) on the serum metabolome and intestinal luminal content of fatty acids in colitic rats. Colitis was induced in rats using trinitrobenzene sulfonic acid. Subsequently, rats received intragastric administration of either 250 mg LS/kg body weight or saline (the control group) every day for 5 weeks. Short-chain fatty acids in the intestinal lumen, blood profile, and metabolites in serum were measured, respectively, using gas chromatography, biochemistry analyzer, and nuclear magnetic resonance-based metabolomics combined with multivariate statistics. Metabolic effects of LS included: (1) decreases in concentrations of branched-chain amino acids (isoleucine and valine), alanine, citric acid, trimethylamine oxide and taurine, and the abundance of aspartate aminotransferase in serum; (2) increases in concentrations of glucose metabolites (including succinate) in serum; and (3) altered concentrations of butyrate in the cecal content and of butyrate and acetate in the colon content. The results indicate that LS supplementation to colitic rats affects whole-body metabolism of amino acids and release of aspartate aminotransferase and alkaline phosphatase from tissues into the blood circulation, and enhances the production of short-chain fatty acids in the intestinal lumen. |
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