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| 转基因棉花外源基因的遗传 | |
| 引用本文： | 王清连,张宝红,郭腾龙,许欣然. 转基因棉花外源基因的遗传[J]. 生命科学研究, 2001, 5(4): 345-350 |
| 作者姓名： | 王清连 张宝红 郭腾龙 许欣然 |
| 作者单位： | 1. 河南职业技术师范学院, 2. 中国农业科学院棉花研究所, 3. 山东荷泽农业科学研究所, |
| 基金项目： | 河南省自然科学基金项目(984011000) |
| 摘 要： | 选用不同来源的转Bt基因抗虫棉中棉所30号和新棉33B、转tfdA基因抗除草剂棉花材料TFD，通过与不同主栽推广品种正反交，研究了外源Bt基因和tfdA基因在棉株体内的遗传和分离行为。结果表明，由于外源基因提供给转基因棉花的抗虫和抗除草剂性状均是由一对于基因控制的显性性状，且该性状不受细胞质效应的影响，符合孟德尔遗传规律。 |
| 关 键 词： | 转基因棉花 Bt基因 tfdA基因 遗传 显性性状 |
| 文章编号： | 1007-7847(2001)04-0345-06 |
| 修稿时间： | 2001-06-19 |
lnheritance of Exogenous Genes in Transgenic Cotton |
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| WANG Qing lian ,ZHANG Bao hong ,GUO Teng long ,XU Xin ran. lnheritance of Exogenous Genes in Transgenic Cotton[J]. Life Science Research, 2001, 5(4): 345-350 | |
| Authors: | WANG Qing lian ZHANG Bao hong GUO Teng long XU Xin ran |
| Affiliation: | WANG Qing lian 1,ZHANG Bao hong 2,GUO Teng long 3,XU Xin ran 1 |
| Abstract: | Three transgenic cotton varieties(lines)were chosen for the study of inheritance of foreign Bt ( Bacillusthuringiensis toxin )and tfdA genes in cotton The transformed cotton varieties CCRI 30 and NewCott 33B expressing the BtcryIA gene,and cotton 1ine TFD expressing the tfdA gene were crossed with CCRI 19,CCRI 12 and Lumain 6 The results confirm inheritance and segregation of (i) the exogenous Bt gene in transgenic CCRI 30 and NewCott 33 B, governing resistance to bollworm,and (ii) the exogenous tfdA gene in transgenic TFD,governing resistance to the herbicide 2,4 D Both resistance characters were governed by a single dominant nuclear gene, and were not affected by cytoplasm Our data support the conclusion that foreign traits encoded by single genes are inherited and expressed in Mendelian fashion in cotton Our results also indicate that a practical backcross breeding program could be used to develop cotton cultivars combining one or more resistance traits from foreign and native gene sources |
| Keywords: | transgenic cotton Bt gene tfdA gene inheritance dominant gene |
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