The expression of I-Ed molecules in F1 hybrid mice detected with antigen-specific,I-Ed-restricted cloned T-cell lines |
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Authors: | Patricia J Conrad Charles A Janeway Jr |
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Institution: | (1) Department of Pathology, Howard Hughes Medical Institute, Yale University School of Medicine, 06510 New Haven, Connecticut |
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Abstract: | The expression of polymorphic determinants on I-E molecules is largely dependent on allelic variation in the E
chain. We have previously analyzed the expression of E
k
and E
b
chains in F1 hybrid mice by a combination of techniques, and have shown that functional variation detected by the responsiveness of cloned T-cell lines specific for these molecules correlates well with serological determination of E
expression. In the present study, we have extended our analysis to E
d
expression in F1 hybrid mice. We show that E
d
is relatively poorly expressed in three F1 combinations: H-2
d× H-2
b, H-2
d× H-2
s, and H-2
d× H-2
u. The former two crosses express E
chains from the H-2
dparent only; when recombinant strains carrying E
b
or E
s
and an active E
gene are used, E
d
expression is significantly increased. On the other hand, H-2
umice synthesize E
chains; the poor expression of E
d
chains in this F1 hybrid apparently reflects the strong preferential association of E
u
chains with all E
molecules thus far analyzed. These results confirm that E
chains compete for binding to E
chains and that preferential association of different allelic forms of E
chains with E
chains is a generalized phenomenon. They also illustrate the importance of the rate of biosynthesis of Ia chains for cell-surface expression. |
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Keywords: | |
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