Glycogen-bound polyphosphate kinase from the archaebacterium Sulfolobus acidocaldarius. |
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Authors: | R Sk rko, J Osipiuk, K O Stetter |
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Affiliation: | Lehrstuhl für Mikrobiologie, Universit?t Regensburg, Federal Republic of Germany. |
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Abstract: | Glycogen-bound polyphosphate kinase has been isolated from a crude extract of Sulfolobus acidocaldarius by isopycnic centrifugation in CsCl. Divalent cations (Mn2+ greater than Mg2+) stimulated the reaction. The enzyme does not require the presence of histones for its activity; it is inhibited strongly by phosphate and slightly by fluoride. The protein from the glycogen complex migrated in a sodium dodecyl sulfate-polyacrylamide gel as a 57-kilodalton protein band; after isoelectric focusing it separated into several spots in the pH range of 5.6 to 6.7. |
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