Cloning and sequence analyses of a 2,3-dihydroxybiphenyl 1,2-dioxygenase gene (<Emphasis Type="Italic">bphC</Emphasis>) from<Emphasis Type="Italic"> Comamonas</Emphasis> sp. SMN4 for phylogenetic and structural analysis期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Cloning and sequence analyses of a 2,3-dihydroxybiphenyl 1,2-dioxygenase gene (bphC) from Comamonas sp. SMN4 for phylogenetic and structural analysis

Authors:	N.?R.?Lee,D.?Y.?Kwon author-information" > author-information__contact u-icon-before" > mailto:dykwon@kfri.re.kr" title=" dykwon@kfri.re.kr" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author,K.?H.?Min

Affiliation:	(1) Department of Biological Science, Sookmyung Women's University, Chungpa 2-ka, Yongsan, 140–742, Seoul, Korea,;(2) , Korea Food Research Institute, Baekhyon, Poondang, Songnam, 463–746, Kyongki-do, Korea,

Abstract:	A genomic library of biphenyl-degrading Comamonas sp. SMN4 for isolating fragments containing the 2,3-dihydroxybiphenyl 1,2-dioxygenase (23DBDO) gene was constructed. The smallest subclone (pNPX9) encoding 23DBDO activity was sequenced and analyzed. The C-terminal domain of 23DBDO from Comamonas sp. SMN4 had five catalytically essential residues and was more highly conserved than the N-terminal domain. Phylogenetic and structural relationships of 23DBDO from Comamonas sp. SMN4 were analyzed. Electronic Publication

Keywords:	Dihydroxybiphenyl dioxygenase bphC Extradiol dioxygenase Comamonas Biphenyl degradation
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