A convenient method for preparative peptide separation

A chromatographic system has been developed for the separation of peptides on a preparative scale. The principle of the method is to use the peptide bond absorbance in the 200–250 nm wavelength range as the basis for the peptide detection, and therefore to use only inorganic buffers that are transparent at these low wavelengths. Chromatography on phosphocellulose and on triethylaminoethyl-(TEAE-)cellulose leading to complete separation of the tryptic peptides of oxidized pancreatic ribonuclease is reported as an illustration of the method. The new system, which can be used both rapidly and conveniently and with simple equipment, appears to have broad applicability to peptide separation.