轮状病毒VP7基因在大肠杆菌中的表达及其免疫原性

轮状病毒是世界范围内引起婴幼儿病毒性腹泻的主要病原体。VP7是轮状病毒的主要外壳蛋白和中和抗原，是发展基因工程疫苗的首选。把包含全部3个主要抗原性区域的轮状病毒SA11 VP7基因片段以谷胱甘肽S转移酶融合蛋白的形式在大肠杆菌中进行表达，表达产物占菌体总蛋白的30%左右。经一步Glutathione Sepharose4B亲和纯化，重组蛋白纯度超过90%。Western blot实验表明，重组蛋白可被抗SA11的多抗特异地识别。动物实验表明，重组抗原可在小鼠和家兔体内诱导VP7特异的抗体和一定水平的SA11中和抗体。

Expression in Escherichia coli and Imnunogenicity of Rotavirus VP7

Rotaviruses are the single most important cause of severe diarrhea in young children all over the world.VP7 is the major outer capsid and is a primary candidate for inclusion in a subunit or recombinant vaccine.Part of the VP7 gene containing all the three antigenic regions was expressed as a chimeric protein with glutathione S\|transferase (GST) in \%E.coli.\%The chimeric protein,representing about 30% of the total protein of the recombinant\|plasmid\|carrying bacteria,reacted with polyclonal antibodies raised against whole virus.Immunization of sero\|negative rabbits and mice with purified fusion\|protein generated both virus\|binding and neutralizing antibodies.