Mapping of genetic modifiers affecting the eye phenotype of ocular retardation (Chx10
or-J
) mice |
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Authors: | Gilbert Wong Sharmila Basu Conger Margit Burmeister |
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Institution: | (1) Molecular and Behavioral Neuroscience Institute, University of Michigan, 205 Zina Pitcher Place, Ann Arbor, Michigan 48109, USA;(2) Present address: State Higher Education Executive Officers, Boulder, Colorado |
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Abstract: | Ocular retardation is a recessive murine mutation whose phenotypic expression is greatly affected by genetic background effects.
Mice of the inbred 129/SvJ background that are homozygous for the Chx10or-J mutation are blind and have a thin, poorly differentiated retina and no optic nerve. A backcross between 129/SvJ and Mus musculus castaneus (CASA/Rk) produced animals that were homozygous for the Chx10or-J mutation, yet showed a much milder phenotype. Such animals, when brother-sister mated and selected for mild phenotype for
several generations, resulted in partial recovery of visual function, including presence of an optic nerve and pupillary response.
In this article we report a genome scan of phenotypic extremes of the backcross to identify the genetic loci affecting this
phenotype modification. Our scan revealed significant loci on Chromosomes 6 and 14 where the CASA/Rk alleles are maintained
selectively. Markers were developed near candidate genes, but no candidate gene could be identified unequivocally.
Electronic Supplementary Material Electronic Supplementary material is available for this article at and accessible for authorised users. |
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Keywords: | |
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