Incorporation of a lambda phage recombination system and EGFP detection to simplify mutagenesis of Herpes simplex virus bacterial artificial chromosomes |
| |
Authors: | Falko Schmeisser Jerry P Weir |
| |
Institution: | (1) Laboratory of DNA Viruses, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA |
| |
Abstract: | Background Targeted mutagenesis of the herpesvirus genomes has been facilitated by the use of bacterial artificial chromosome (BAC) technology.
Such modified genomes have potential uses in understanding viral pathogenesis, gene identification and characterization, and
the development of new viral vectors and vaccines. We have previously described the construction of a herpes simplex virus
2 (HSV-2) BAC and the use of an allele replacement strategy to construct HSV-2 recombinants. While the BAC mutagenesis procedure
is a powerful method to generate HSV-2 recombinants, particularly in the absence of selective marker in eukaryotic culture,
the mutagenesis procedure is still difficult and cumbersome. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|