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Bioreactor for solid-state cultivation of <Emphasis Type="Italic">Phlebiopsis gigantea</Emphasis>
Authors:Veera Virtanen  Antti Nyyssölä  Antti Vuolanto  Matti Leisola  Pekka Seiskari
Institution:(1) Laboratory of Bioprocess Engineering, Helsinki University of Technology, P.O. Box 6100, 02015 Espoo, Finland;(2) Present address: Oy Keskuslaboratorio – Centrallaboratorium Ab, P.O. Box 70, 02151 Espoo, Finland;(3) Present address: Mobidiag Ltd, Haartmaninkatu 8/Biomedicum, 00290 Helsinki, Finland;(4) Verdera Oy, PL 5, 02271 Espoo, Finland
Abstract:Phlebiopsis gigantea fungus used in biological control of root rot is currently cultivated commercially in disposable, sterilizable plastic bags. A novel packed bed bioreactor was designed for cultivating P. gigantea and compared to the plastic bag method and to a tray bioreactor. The spore viability of 5.4 × 106 c.f.u./g obtained with the packed bed bioreactor was of the same order of magnitude as the viabilities obtained with the other cultivation methods. Furthermore, the packed bed bioreactor was less time and space consuming and easier to operate than the tray bioreactor.
Keywords:Biofungicides  Packed bed bioreactor            Phlebiopsis gigantea            Solid-state cultivation  Tray bioreactor
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