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羊草根茎的贮藏碳水化合物及对氮素添加的响应
引用本文:潘庆民,白永飞,韩兴国,张丽霞. 羊草根茎的贮藏碳水化合物及对氮素添加的响应[J]. 植物生态学报, 2004, 28(1): 53-58. DOI: 10.17521/cjpe.2004.0008
作者姓名:潘庆民  白永飞  韩兴国  张丽霞
作者单位:中国科学院植物研究所植被数量生态学重点实验室,北京,100093
基金项目:中国科学院知识创新工程项目,国家自然科学基金
摘    要: 为了研究氮素对羊草(Leymus chinensis)根茎碳水化合物贮藏的影响,在中国科学院内蒙古草原生态系统定位研究站的羊草样地,设计了不同水平和不同时期的氮素添加试验。采用高效液相色谱(High Performance Liquid Chromatography, HPLC)对羊草根茎中的贮藏性碳水化合物进行了测定。结果表明,羊草根茎中的贮藏碳水化合物组分包括果聚糖、甘露糖醇、蔗糖、葡萄糖和果糖。其中果聚糖是最主要贮藏碳水化合物,约占60%;其次是甘露糖醇,约占20%。氮素添加量对羊草根茎中的贮藏碳水化合物有显著影响。在0~17.5 g N·m-2范围内,随着氮素添加量的增加,碳水化合物总量、果聚糖、甘露糖醇的含量均逐渐升高。氮素添加时期对羊草根茎中的贮藏碳水化合物的含量亦有显著影响。在7月初添加氮素比4月份添加氮素更有利于贮藏碳水化合物的积累。 关键词

关 键 词:根茎  贮藏营养  果聚糖  甘露糖醇  氮素水平
修稿时间:2002-09-12

CARBOHYDRATE RESERVES IN THE RHIZOME OF LEYMUS CHINENSIS IN RESPONSE TO NITROGEN ADDITIONS
PAN Qing-Min BAI Yong-Fei HAN Xing-Guo and ZHANG Li-Xia. CARBOHYDRATE RESERVES IN THE RHIZOME OF LEYMUS CHINENSIS IN RESPONSE TO NITROGEN ADDITIONS[J]. Acta Phytoecologica Sinica, 2004, 28(1): 53-58. DOI: 10.17521/cjpe.2004.0008
Authors:PAN Qing-Min BAI Yong-Fei HAN Xing-Guo and ZHANG Li-Xia
Abstract:Carbohydrates serve as food reserves for grasses at times when photosynthesis cannot supply sufficient carbon for maintenance and growth such as when grasses are completely defoliated during the winter and early spring. L. chinensis, a rhizomatous graminoid that commonly dominates the steppe in semiarid areas of northern China, stores enough carbohydrates in the rhizome before winter to ensure that it can survive the hard environmental conditions of the winter. Nitrogen (N) fertilization is a common technique used to increase forage productivity in many countries; however, fertilization is not widely used in the steppe grasslands of northern China. Nitrogen fertilization significantly affects the non-structural carbohydrates during both growth and dormant periods of some grass species, but the effect of N fertilization on carbohydrate storage in the L. chinensis rhizome remains unclear. We conducted an N addition experiment with different rates and different application times of N to L. chinensis in fenced plots at the Inner Mongolia Grassland Ecosystem Research Station, Chinese Academy of Sciences. In the first experiment, NH4NO3 was applied at six treatment levels (0, 1.75, 5.25, 10.5, 17.5, 28 g N·m-2) on July 5 every year. In the second experiment, the timing of N application was studied by applying 17.5 g N·m-2 NH4NO3 on two dates, April 20 and July 5. In both experiments, 9 replicate plots (5 m x 5 m) were used for each treatment in a complete randomly designed experiment. L. chinensis rhizome samples were collected from the field on Sep. 20. Rhizomes were washed with cold water, oven-dried at 80 ℃ for 24 hours and ground to pass through a 100 μm mesh screen. Stored carbohydrates in the rhizome of L. chinensis were analyzed using a Sugar-PAKTM I column, a specific column for carbohydrate components assay, in High Performance Liquid Chromatography (HPLC). The results showed that the stored carbohydrates of the L. chinensis rhizome consisted of fructan, mannitol, sucrose, fructose, and glucose. Fructan, which contributed 60% of total non-structural carbohydrates (TNC), was the predominant stored carbohydrate followed by mannitol, which contributed 20% of TNC. Both fructose and glucose were less than 15% of TNC and sucrose was only 6% of TNC. Hence, sucrose, a main component of stored carbohydrates in other grasses, was only a minor component of L. chinensis. Nitrogen rates significantly influenced the contents of fructan, mannitol and total carbohydrates, but had little effects on glucose, fructose and sucrose content in the L. chinensis rhizome. The total carbohydrate, fructan and mannitol concentrations increased in the rhizome with increasing N application rates from 0 to 17.5 g N·m-2, but carbohydrate reserves were reduced at application rates of 28 g N·m-2. The timing of N application also significantly influenced the contents of carbohydrate reserves; L. chinensis stored more carbohydrates in the rhizome when N was applied in July than that in April. In conclusion, fructan and mannitol are important stored carbohydrates in the rhizome of L. chinensis. Both N application rates and timing of application significantly affect the levels of carbohydrate reserves, especially fructan and mannitol in the L. chinensis rhizome.
Keywords:Leymus chinensis   Rhizome   Carbohydrates   Fructan   Mannitol   Nitrogen fertilization  
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