Isolation and characterization of microsatellite loci in <Emphasis Type="Italic">Taxus sumatrana</Emphasis> (Taxaceae) using PCR-based isolation of microsatellite arrays (PIMA) |
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| Authors: | Chi-Chun Huang Tzen-Yuh Chiang Tsai-Wen Hsu |
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| Institution: | (1) Department of Life Sciences, Cheng Kung University, Tainan, 701, Taiwan, Republic of China;(2) Department of Botany, Taiwan Endemic Species Research Institute, Nantou, 552, Taiwan, Republic of China |
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| Abstract: | Taxus sumatrana (Miq.) de Laub. (Taxaceae) is an endangered conifer with a scattered distribution in central Taiwan. In this study, we described
the development of 12 microsatellite loci in T. sumatrana for genetic studies. These new markers were tested in nine individuals of the rare species. The number of alleles ranged
from 3 to 13 and expected heterozygosity from 0.627 to 0.948. Eleven of twelve loci are significantly deviated from Hardy–Weinberg
expectations due to the heterozygote deficiency. |
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| Keywords: | Microsatellite PIMA RAPD-PCR enrichment Taxus sumatrana |
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