Determination of α- and β-trenbolone in bovine muscle and liver by liquid chromatography with fluorescence detection |
| |
Authors: | Naoki Yoshioka Yumi Akiyama Nobuyuki Takeda |
| |
Institution: | Hyogo Prefectural Institute of Public Health, 2-1-29 Arata-cho, Hyogo-ku, Kobe 652-0032, Japan |
| |
Abstract: | A sensitive and rapid high-performance liquid chromatographic screening method is described for the determination of anabolic steroid trenbolone in bovine muscle and liver. Trenbolone was analyzed as α- and β-trenbolone. Samples were extracted with ethyl acetate and cleaned up on a silica solid-phase extraction (SPE) cartridge. Liver samples were cleaned up on a multifunctional SPE cartridge before using a silica SPE cartridge. Analysis of α- and β-trenbolone was performed by reversed-phase high-performance liquid chromatography (HPLC) with a fluorescence detector. The detection limits for this method were estimated to be 0.2 and 1.0 ng/g in bovine muscle and liver, respectively. The mean recoveries spiked in muscle at 2 ng/g and in liver at 10 ng/g were over 80%. |
| |
Keywords: | Trenbolone |
本文献已被 ScienceDirect 等数据库收录! |