High-performance liquid chromatographic assay for minocycline in human plasma and parotid saliva |
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Authors: | Valerie Orti Michel Audran Philippe Gibert Guillaume Bougard Franoise Bressolle |
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Institution: | a Laboratoire de Pharmacocinétique, Faculté de Pharmacie, 34060 Montpellier Cedex 2, France;b Département de Biophysique, Faculté de Pharmacie, 34060 Montpellier Cedex 2, France;c Faculté d’Odontologie, 34295 Montpellier Cedex 5, France |
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Abstract: | A sensitive and specific high-performance liquid chromatographic (HPLC) method with UV detection was developed for the determination of minocycline in human plasma and parotid saliva samples. Samples were extracted using an Oasis™ HLB cartridge and were injected into a C8 Nucleosil column. The HPLC eluent contained acetonitrile–methanol–distilled water–0.1% trifluoroacetic acid (25:2:72.9:0.1, v/v). Demeclocycline was used as internal standard. The assay showed linearity in the tested range of 0.1–25 μg/ml. The limit of quantitation was 100 ng/ml. Recovery from plasma or parotid saliva averaged 95%. Precision expressed as %CV was in the range 0.2–17% (limit of quantitation). Accuracy ranged from 93 to 111%. In the two matrices studied at 20 and 4°C, rapid degradation of the drug occurred. Frozen at −30°C, this drug was stable for at least 2 months, the percent recovery averaged 90%. The method’s ability to quantify minocycline with precision, accuracy and sensitivity makes it useful in pharmacokinetic studies. |
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Keywords: | Minocycline |
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